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M9480207.TXT
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1994-08-09
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Document 0207
DOCN M9480207
TI Defective infection of rabbit peripheral blood monocyte cultures with
human immunodeficiency virus type 1.
DT 9410
AU Tseng CK; Leibowitz J; Sell S; Department of Pathology and Laboratory
Medicine, Medical School,; University of Texas Health Science Center at
Houston 77225.
SO AIDS Res Hum Retroviruses. 1994 Mar;10(3):285-93. Unique Identifier :
AIDSLINE MED/94289067
AB Human immunodeficiency virus type 1 (HIV-1) produces abortive infections
of primary cultures of rabbit peripheral blood mononuclear cells
(PMBCs). Mitogen activation of rabbit PBMCs or the addition of exogenous
cytokines to the cultures does not change the level of release of the
early HIV core protein, p24, into the culture medium. The amount of p24
increases steadily and reaches peak levels about 7 days after infection.
HIV-1-specific DNA env sequences are also detected in infected PBMCs.
However, reverse transcription of RNA of samples from activated infected
cultures to cDNA, followed by amplification by the polymerase chain
reaction, revealed transcription of gag, but not env, regions,
suggesting that HIV-1 infection of rabbit PBMC does not lead to the
replication and maturation of complete HIV-1 virions. In addition,
neither CPE nor lytic infection was observed in HIV-1-infected rabbit
cells and infectivity could not be transferred from rabbit cells
infected with HIV for up to 2 weeks to MT-2 or H9 indicator cell lines.
In addition, no CPE was seen in long-term cultures of the
HTLV-I-transformed rabbit cell line PLT-1441, after inoculation with
HIV. It is concluded that primary rabbit PBMCs may be infectable by
HIV-1 but are not permissive for production of infectious virus. This
conclusion is consistent with the apparent long-term latent infection
seen after inoculation of rabbits with HIV-1 or HIV-1-infected cells.
DE Animal Cell Line Cells, Cultured Cytopathogenic Effect, Viral
Defective Viruses Human HIV Core Protein p24/BIOSYNTHESIS
HIV-1/*PHYSIOLOGY Kinetics Leukocytes, Mononuclear/*MICROBIOLOGY
Rabbits Support, Non-U.S. Gov't Virus Replication JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).